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Determination of thiocyanate in plasma by ion chromatography and ultraviolet detection.
Content Provider | Semantic Scholar |
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Author | Saussereau, Élodie Goullé, Jean-Pierre Lacroix, Christian |
Copyright Year | 2007 |
Abstract | A specific and sensitive rapid high-performance liquid chromatographic method has been developed for determination of thiocyanate (SCN-) in plasma. This method is based on anion exchange chromatography after a simple ultrafiltration of plasma diluted in water. The detection of SCN- is carried out in ultraviolet (lambda = 210 nm). The proposed method is linear in the range 1-30 mg/L. Intra-assay and interassay accuracy and precision were maintained within the designated limit (< 20%). The total recovery of SCN- varied between 97 and 103.9%. The method described should be useful for clinical medicine. Moreover, this method was applied to the analysis of SCN- plasma in deceased subjects, within the context of fire, and could be of interest in forensic science as a useful additional measurement tool for cyanide determination in blood. |
File Format | PDF HTM / HTML |
DOI | 10.1093/jat/31.7.383 |
Alternate Webpage(s) | https://watermark.silverchair.com/31-7-383.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAjwwggI4BgkqhkiG9w0BBwagggIpMIICJQIBADCCAh4GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMCthLI2VM2o6jdMuEAgEQgIIB7-HF6HuOC2jtPH4_NWLPKaYhByGs5YxVX0Q2-0Tfzcs6upYfzdPOyWXwvU56E2NErgvOHQ7zu8zp9skdUNaINSQdueh9nW8Xcaktka8uD7PjB4cZwm53onMAx0hJV9jdEredRsDPtGjIhWTzQiAdBN7fZNrp4-rGZetRHpMx0T7rZVW8o3Avls7rJZh4draRKy-rBOf0Nt-bI8xWhDOszf9yMhLtZbpLQFdIlj8r8q5pWrd8XCbsPAw3vA0nKwmS3Lr6kZ3BiUyJtdzSf-xlBA2UaGU47k5CA9Max-OnzcIYIdu44gkF7gIDMG1R5xztZKrtHfDAv5G0ooHNehmEyxk1p2LRs51jmTjYxSo6wyS2ovcZrKlv9im6Ndl6eMcoEX51e_ZCdUbF4ZnYw80EXBaxLlEkgcOpRZOWHBoeZUi97zFQfZNcV5M3pZ3K8_6Qiu70hii2QBZp12U6YaH1kNgpr-buktxlmDecycAOiiZHG6kzs6frdiaW5m4ITVENlYnoQWsziMQtykcRanG6B-V-G9kY34J_mmLcs7xs5kVUneimQTccaH2ZV4NosXYGsx2nnMAjVqN_iaKmMm4Y5Z3FR9Hhdtn8hBIUJbdjrjYOGgu6LzMEtdikPu8eiGpfHlo7ZhGd1f_HyeDRPxfHEA |
PubMed reference number | 17725886 |
Alternate Webpage(s) | https://doi.org/10.1093/jat%2F31.7.383 |
Volume Number | 31 |
Issue Number | 7 |
Journal | Journal of analytical toxicology |
Language | English |
Access Restriction | Open |
Content Type | Text |
Resource Type | Article |