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Thiocyanate determination by ion pair chromatography
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tamošiūnas, Vytautas Padarauskas, Audrius Pranaitytė, Birutė |
| Copyright Year | 2006 |
| Abstract | A simple and rapid ion pair chromatographic method has been developed for the determination of thiocyanate. Separation was carried out on a 25 cm × 4.6 mm Ascentis C 18 column (5 µm particle size) with a mobile phase containing 2 mmol/l tetrabutylammonium (TBA)-phosphate (pH 6.5) in 40 (v/v) % acetonitrile at a flow rate of 0.5 ml/min. Detection was performed by direct UV absorption at 210 nm. This study investigated the effect of various experimental factors, such as acetonitrile content, pH, and ion-pairing cation concentration in the mobile phase on the retention behaviour of anions. Under optimized conditions, four anions (Br , NO 3 , I and SCN ) were effectively separated within 8 minutes. The calibration curve was linear in the thiocyanate concentration range from 5×10 -6 to 5×10 -4 mol/l with an excellent correlation coefficient of more than 0.999. The limit of detection was 1.5×10 -6 mol/l (signal / noise = 3). The method was applied for determination of thiocyanate ions in human saliva. Saliva sample preparation involved only centrifugation and appropriate dilution. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.elibrary.lt/resursai/LMA/Chemija/0623_Che_021_024.pdf |
| Alternate Webpage(s) | https://epublications.vu.lt/object/elaba:6172961/6172961.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
