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Effects of growth hormone antagonists on 3 T 3F 442 A preadipocyte differentiation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chen, Wei Yu Wiehl, Peter Okada, Sumiyo Kopchick |
| Copyright Year | 1997 |
| Abstract | We have previously shown that a bovine (b) GH antagonist, bGH-M8, which possesses three amino acid substitutions in its third a-helix, inhibits mouse 3T3-F442A preadipocyte differentiation. In the current studies, we used the bGH and human (h) GH analogs with single amino acid substitution, bGH-GI 19R and hGH-G120R, for determining their biological activity using the preadipocyte hfferentiation assay. Short-term and long-term GH-inducible events were studied during adipose differentiation, including late marker gene expression (adipocyte protein 2), immediate early gene induction (c-fos), and tyrosine phosphorylation of intracellular proteins. The results demonstrated that these GH analogs not only failed to induce these three events, but also antagonized GH induction of c-fos expression and phosphorylation of proteins of apparent molecular mass of 95 kDa. Our present study agrees with the notion that GH must bind to the GH receptor via site one and with a second GH receptor molecule (or with some yet unidentified 'second target') through GH binding site two. This interaction is important for subsequent GH-dependent biological events. lournal of Endocrinology (1 995) 146, 13 1-1 39 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cheserver.ent.ohiou.edu/Paper-gu/JJK-HGH.PDF |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
