Loading...
Please wait, while we are loading the content...
Similar Documents
Growth hormone and phorbol esters require specific protein kinase C isoforms to activate mitogen-activated protein kinases in 3 T 3F 442 A cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mackenzie, Simon Fleming, I. Anderson, Neil G. Kilgour, Elaine |
| Copyright Year | 1997 |
| Abstract | Previous studies have shown that the activation of p44 and p42 mitogen-activated protein (MAP) kinases (ERK1 and ERK2) by growth hormone (GH) and phorbol esters, but not by epidermal growth factor, in 3T3-F442A preadipocytes is dependent on protein kinase C (PKC). In the present study two approaches have been taken to determine the PKC isoform dependence of MAP kinase activation in these cells. By immunoblotting with specific antibodies, the cells were found to express PKC-α, -γ, -δ, -ε and -ζ. Treatment of cells with 500 nM PMA for 3 h led to the complete depletion of PKC-δ and the partial depletion of PKC-α but did not significantly affect the expression of the other PKC isoforms. In parallel, such treatment severely attenuated the ability of GH to activate MAP kinase. The degree of this attenuation was not increased by more prolonged PMA pretreatment, indicating that PKC-δ and perhaps PKC-α are important for MAP kinase activation by GH. These experiments further revealed that additional PKC isoforms were required for |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/324/1/159.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
