Loading...
Please wait, while we are loading the content...
Similar Documents
A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hirayama, Tasuku Okuda, Kensuke Nagasawa, Hideko |
| Copyright Year | 2013 |
| Abstract | Although labile iron plays critical roles in diverse biological processes in living cells, the physiological and pathophysiological functions of iron have not been sufficiently explored, partially due to a lack of methods for visualizing intracellular labile iron. In this edge article, we present a novel turn-on fluorescent probe (RhoNox-1) for the selective detection of Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations and electrochemical studies revealed that fluorescence quenching of RhoNox-1 occurred in physiological conditions, which was attributed to non-radiative deactivation of the excited state of tertiary amine N-oxide substituted xanthene involving a twisted internal charge transfer (TICT) process and partially due to photo-induced electron transfer (PET) from the N-oxide group. RhoNox-1 showed significant enhancement of the fluorescence signal in Fe2+-loaded cells via selective Fe2+-mediated deoxygenation of the N-oxide group and also successfully detected basal and endogenous labile Fe2+ in living cells. |
| Starting Page | 1250 |
| Ending Page | 1256 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/C2SC21649C |
| Volume Number | 4 |
| Alternate Webpage(s) | http://www.rsc.org/suppdata/sc/c2/c2sc21649c/c2sc21649c.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/C2SC21649C |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
