NDLI: A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems.

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A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems.

Content Provider	Semantic Scholar
Author	Long, Lingliang Wang, Ning Han, Yuanyuan Huang, Meiyu Yuan, Xiangqi Cao, Siyu Gong, Aihua Wang, Kun
Copyright Year	2018
Abstract	Labile Fe2+ has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe2+ in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe2+. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe2+ in living systems based on a novel Fe2+ meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe2+, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe2+, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe2+ in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe2+ in living systems were also successfully monitored.
Starting Page	2555
Ending Page	2562
Page Count	8
File Format	PDF HTM / HTML
PubMed reference number	29721571
Journal	Medline
Volume Number	143
Issue Number	11
Alternate Webpage(s)	http://www.rsc.org/suppdata/c8/an/c8an00556g/c8an00556g1.pdf
Alternate Webpage(s)	https://doi.org/10.1039/c8an00556g
Journal	The Analyst
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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