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Variant estrogen receptor-alpha messenger RNA expression in hormone-independent human breast cancer cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Coutts, Amanda S. Leygue, Etienne Murphy, Leigh C. |
| Copyright Year | 1999 |
| Abstract | T5-PRF cells are insensitive to the growth-stimulatory effects of estrogen while still retaining expression of estrogen receptor-alpha (ER-alpha). In the apparent absence of ligand, T5-PRF cells have a 3. 6+/-0.5 (s.e.m.)-fold increased basal ER-alpha activity and elevated basal progesterone receptor levels compared with the parent, estrogen-sensitive, T5 cells. Long-range ER-alpha reverse transcription-PCR was performed to characterize variant ER-alpha mRNA expression in the two cell lines. An increased relative expression of an exon 3/4-deleted ER-alpha mRNA variant was found in T5-PRF. Recombinant expression of this ER-alpha variant resulted in significantly increased estrogen responsiveness, as well as a trend to increased basal ligand-independent activity when expressed with wild-type ER-alpha in ER-negative cell lines, as well as significantly increasing both ligand-independent and estrogen-induced ER-alpha transcriptional activity when expressed in parental T5 cells. These results suggest a role for altered variant ER-alpha in ligand-independent activation of ER-alpha which may contribute to hormone independence in breast tumors. |
| Starting Page | 2115 |
| Ending Page | 2118 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://jme.bioscientifica.com/downloadpdf/journals/jme/23/3/325.pdf |
| Alternate Webpage(s) | http://intl-jme.endocrinology-journals.org/content/23/3/325.full.pdf |
| PubMed reference number | 10601978v1 |
| Volume Number | 23 |
| Issue Number | 3 |
| Journal | Journal of molecular endocrinology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adrenergic alpha-Agonists Cultured Cell Line Estrogens Glycoprotein Hormones, alpha Subunit Ligands Mammary Neoplasms Progesterone Reverse Transcription Transcription, Genetic cancer cell estrogen receptor alpha, human negative regulation of RNA-directed RNA polymerase activity |
| Content Type | Text |
| Resource Type | Article |
