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Selective coactivation of estrogen-dependent transcription by CITED1 CBP/p300-binding protein.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yahata, Tetsuro Shao, Weijian Endoh, Hiroshi Hur, Jong Hyun Ueda, Yasuaki Kato, Shigeaki Isselbacher, Kurt J. Brown, Myles Shioda, Toshishiro |
| Copyright Year | 2001 |
| Abstract | CITED1, a CBP/p300-binding nuclear protein that does not bind directly to DNA, is a transcriptional coregulator. Here, we show evidence that CITED1 functions as a selective coactivator for estrogen-dependent transcription. When transfected, CITED1 enhanced transcriptional activation by the ligand-binding/AF2 domain of both estrogen receptor-alpha (ERalpha) and ERbeta in an estrogen-dependent manner, but it affected transcriptional activities of other nuclear receptors only marginally. CITED1 bound directly to ERalpha in an estrogen-dependent manner through its transactivating domain, and this binding activity was separable from its p300-binding activity. CITED1 was strongly expressed in nulliparous mouse mammary epithelial cells and, when expressed in ER-positive MCF-7 breast cancer cells by transduction, exogenous CITED1 enhanced sensitivity of MCF-7 cells to estrogen, stabilizing the estrogen-dependent interaction between p300 and ERalpha. The estrogen-induced expression of the transforming growth factor-alpha (TGF-alpha) mRNA transcript was enhanced in the CITED1-expressing MCF-7 cells, whereas estrogen-induced expression of the mRNA transcripts for progesterone receptor or pS2 was not affected. Chromatin immunoprecipitation assay revealed that endogenous CITED1 is recruited to the chromosomal TGF-alpha promoter in MCF-7 cells in an estrogen-dependent manner but not to the pS2 promoter. These results suggest that CITED1 may play roles in regulation of estrogen sensitivity in a gene-specific manner. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://research4.dfci.harvard.edu/brownlab//pdf_files/2001_Yahata.pdf |
| PubMed reference number | 11581164v1 |
| Volume Number | 15 |
| Issue Number | 19 |
| Journal | Genes & development |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CITED1 gene Estrogen Receptor alpha Estrogen Receptor beta Estrogens Growth Factor Ligands Mammary Neoplasms Nuclear Proteins Progesterone Receptors, Nuclear Role Playing DIsorder TGFA protein, human Transcript Transcription, Genetic Transcriptional Activation Transforming Growth Factors cancer cell estrogen receptor alpha, human transduction type I interferon receptor |
| Content Type | Text |
| Resource Type | Article |
