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Sulphate-ion/sodium-ion co-transport by brush-border membrane vesicles isolated from rat kidney cortex.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Luecke, Hartmut Stange, Gertraud Murer, Heini |
| Copyright Year | 1979 |
| Abstract | Uptake of SO(4) (2-) into brush-border membrane vesicles isolated from rat kindey cortex by a Ca(2+)-precipitation method was investigated by using a rapid-filtration technique. Uptake of SO(4) (2-) by the vesicles was osmotically sensitive and represented transport into an intra-vesicular space. Transport of SO(4) (2-) by brush-border membranes was stimulated in the presence of Na(+), compared with the presence of K(+) or other univalent cations. A typical ;overshoot' phenomenon was observed in the presence of an NaCl gradient (100mm-Na(+) outside/zero mm-Na(+) inside). Radioactive-SO(4) (2-) exchange was faster in the presence of Na(+) than in the presence of K(+). Addition of gramicidin-D, an ionophore for univalent cations, decreased the Na(+)-gradient-driven SO(4) (2-) uptake. SO(4) (2-) uptake was only saturable in the presence of Na(+). Counter-transport of Na(+)-dependent SO(4) (2-) transport was shown with MoO(4) (2-) and S(2)O(3) (2-), but not with PO(4) (2-). Changing the electrical potential difference across the vesicle membrane by establishing different diffusion potentials (anion replacement; K(+) gradient+/-valinomycin) was not able to alter Na(+)-dependent SO(4) (2-) uptake. The experiments indicate the presence of an electroneutral Na(+)/SO(4) (2-)-co-transport system in brush-border membrane vesicles isolated from rat kidney cortex. |
| Starting Page | 391 |
| Ending Page | 401 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.biochemj.org/content/ppbiochemj/182/1/223.full.pdf |
| PubMed reference number | 91368v1 |
| Volume Number | 182 |
| Issue Number | 1 |
| Journal | The Biochemical journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Anions Arabic numeral 0 Cations Gradient Gramicidin Import into cell Ionophores Ions Potassium Sodium Chloride Sodium-Dependent Phosphate Transport Protein 2B Structure of cortex of kidney Tissue membrane Vesicle (morphologic abnormality) cif nucleoside transporter filtration vesicle membrane |
| Content Type | Text |
| Resource Type | Article |
