NDLI: Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine across brush border membrane vesicles from rabbit small intestine.

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Effect of hydrogen ion-gradient on carrier-mediated transport of glycylglycine across brush border membrane vesicles from rabbit small intestine.

Content Provider	Semantic Scholar
Author	Takuwa, Noriko Shimada, Tsutomu Matsumoto, Hiroyuki Himukai, Masayoshi Hoshi, Toshinori
Copyright Year	1985
Abstract	Glycylglycine (Gly-Gly) transport across rabbit small intestinal brush border membrane vesicles was studied in the presence and absence of a Na+ gradient or a pH gradient. The transport was found to be entirely independent of Na+ but significantly stimulated by lowering extravesicular pH (pHo). The maximum stimulation was seen at pHo 5.5, where the uptake rate was about 2-times higher than the control value. FCCP, a protonophore, abolished the stimulating effect of low pHo, and low pHo conditions without a pH gradient did not stimulate the uptake rate. Overshoot uptake of Gly-Gly was observed when a pH gradient of 2 pH units was imposed across the vesicular membrane. Valinomycin-induced inside-negative K+ diffusion potential also had a stimulating effect on the uptake, and fluorescence measurements of vesicular suspensions containing diS-C3-(5) revealed the occurrence of depolarization of the vesicular membranes when Gly-Gly was added to the suspensions. Kinetic study showed that a pH gradient caused a decrease of Kt for Gly-Gly without affecting Vmax. All the data obtained indicate that Gly-Gly transport is independent of Na+, dependent on a H+ gradient, and electrogenic, suggesting the mechanism of cotransport with H+.
Starting Page	77
Ending Page	85
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/jjphysiol1950/35/4/35_4_629/_pdf
PubMed reference number	4068369v1
Volume Number	35
Issue Number	4
Journal	The Japanese journal of physiology
Language	English
Access Restriction	Open
Subject Keyword	Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Fluorescence Glycylglycine Gradient Ions Iontophoresis Kinetics Potassium Small Intestinal Wall Tissue Sodium Stimulation (motivation) Suspensions Tissue membrane Valinomycin Vesicle (morphologic abnormality) brush border membrane delta pH pH:LsCnc:Pt:Bld:Qn viral capsid secondary envelopment
Content Type	Text
Resource Type	Article

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