NDLI: cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8.

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cis-trans isomerization of unsaturated fatty acids: cloning and sequencing of the cti gene from Pseudomonas putida P8.

Content Provider	Semantic Scholar
Author	Holtwick, Rita Meinhardt, Friedhelm Keweloh, Heribert
Copyright Year	1997
Abstract	Transposon mutants of Pseudomonas putida P8 were generated by applying a mini-Tn5 mutagenesis system. The mutants obtained were checked for their ability to tolerate increased temperatures and elevated phenol concentrations. Approximately 5,800 transposon mutants were used to generate a pool of 600 temperature-sensitive strains; one of these strains was identified as being damaged in its ability to perform cis-trans isomerization of fatty acids. A gene library of P. putida P8 was constructed and screened by using as a probe sequences immediately adjacent to the mini-Tn5 insertion. A DNA fragment that complemented the mutation was isolated and cloned. The corresponding gene, termed cti, is located close to the methionine synthase locus (metH) in P. putida P8. A cti-carrying fragment integrated into a plasmid also conferred the ability for cis-trans isomerization to Escherichia coli; the cti gene was completely sequenced, and the amino acid sequence was deduced.
File Format	PDF HTM / HTML
DOI	10.1128/aem.63.11.4292-4297.1997
PubMed reference number	9361416
Journal	Medline
Volume Number	63
Issue Number	11
Alternate Webpage(s)	http://aem.asm.org/content/63/11/4292.full.pdf
Alternate Webpage(s)	https://doi.org/10.1128/aem.63.11.4292-4297.1997
Journal	Applied and environmental microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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