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cis/trans isomerase of unsaturated fatty acids of Pseudomonas putida P8: evidence for a heme protein of the cytochrome c type.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Holtwick, Rita Keweloh, Heribert Meinhardt, Friedhelm |
| Copyright Year | 1999 |
| Abstract | From a pool of 600 temperature-sensitive transposon mutants of Pseudomonas putida P8, 1 strain was isolated that carries a mini-Tn5 insertion within the cytochrome c operon. As a result, genes involved in the attachment of heme to cytochrome c-type proteins are turned off. Accordingly, cytochrome c could not be detected spectrophotometrically. The mutant also exhibited a remarkable reduction of cis-trans isomerization capability for unsaturated fatty acids. Consistent with the genetic and physiological data is the detection of a cytochrome c-type heme-binding motif close to the N terminus of the predicted polypeptide of the cis/trans isomerase (cti) gene (CVACH; conserved amino acids in italics). The functional significance of this motif was proven by site-directed mutagenesis. A possible mechanism of heme-catalyzed cis-trans isomerization of unsaturated fatty acids is discussed. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/aem.65.6.2644-2649.1999 |
| PubMed reference number | 10347055 |
| Journal | Medline |
| Volume Number | 65 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://aem.asm.org/content/65/6/2644.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/aem.65.6.2644-2649.1999 |
| Journal | Applied and environmental microbiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
