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A highly stabilizing silver(I)-mediated base pair in parallel-stranded DNA.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sinha, Indranil Guerra, Célia Fonseca Müller, Jens |
| Copyright Year | 2015 |
| Abstract | The first parallel-stranded DNA duplex with Hoogsteen base pairing that readily incorporates an Ag(+) ion into an internal mispair to form a metal-mediated base pair has been created. Towards this end, the highly stabilizing 6 FP-Ag(+)-6 FP base pair comprising the artificial nucleobase 6-furylpurine (6 FP) was devised. A combination of temperature-dependent UV spectroscopy, CD spectroscopy, and DFT calculations was used to confirm the formation of this base pair. The nucleobase 6 FP is capable of forming metal-mediated base pairs both by the Watson-Crick edge (i.e. in regular antiparallel-stranded DNA) and by the Hoogsteen edge (i.e. in parallel-stranded DNA), depending on the oligonucleotide sequence and the experimental conditions. The 6 FP-Ag(+)-6 FP base pair within parallel-stranded DNA is the most strongly stabilizing Ag(+)-mediated base pair reported to date for any type of nucleic acid, with an increase in melting temperature of almost 15 °C upon the binding of one Ag(+) ion. |
| File Format | PDF HTM / HTML |
| DOI | 10.1002/anie.201411931 |
| PubMed reference number | 25631645 |
| Journal | Medline |
| Volume Number | 54 |
| Issue Number | 12 |
| Alternate Webpage(s) | https://www.uni-muenster.de/imperia/md/content/anorganische_und_analytische_chemie/ak_mueller/mueller_angewandte_2015_supp.pdf |
| Alternate Webpage(s) | https://doi.org/10.1002/anie.201411931 |
| Journal | Angewandte Chemie |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
