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Single-Molecule Force Spectroscopy of an Artificial DNA Duplex Comprising a Silver(I)-Mediated Base Pair.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tan, Xinxin Litau, Stefanie Zhang, Xinxin Müller, Jens |
| Copyright Year | 2015 |
| Abstract | Single-molecule force spectroscopy measurements of a DNA duplex comprising an artificial metal-mediated base pair are reported. The measurements reveal that DNA duplexes comprising one central imidazole:imidazole mispair rupture at lower forces than a related duplex with canonical base pairs only. In contrast, DNA duplexes with one central imidazole-Ag(+)-imidazole base pair (formed by the addition of Ag(+) to the aforementioned duplex with the mispair) rupture at higher forces. These measurements indicate for the first time that the increase in thermal stability of a nucleic acid duplex that is observed upon the formation of a metal-mediated base pair is accompanied by a concomitant mechanical stabilization. In fact, the mechanical stabilization even exceeds the thermal one. This result indicates that nucleic acids with metal-mediated base pairs should be ideal building blocks for rigid functionalized DNA nano-objects. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/acs.langmuir.5b03183 |
| Alternate Webpage(s) | https://www.uni-muenster.de/imperia/md/content/anorganische_und_analytische_chemie/ak_mueller/mueller_langmuir_2015_supp.pdf |
| PubMed reference number | 26421907 |
| Alternate Webpage(s) | https://doi.org/10.1021/acs.langmuir.5b03183 |
| Journal | Medline |
| Volume Number | 31 |
| Issue Number | 41 |
| Journal | Langmuir : the ACS journal of surfaces and colloids |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
