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A novel multiplex PCR system for the detection of virulence associated genes of E. coli O157:H7 from food system
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shylaja R. Puttalingamma V. Batra |
| Copyright Year | 2012 |
| Abstract | In view of the importance E. coli serotype 0157:H7 in human diseases, an easy and quick system is desirable to detect toxin producing strains. In this report, we describe standardization of a novel multiplex Polymerase Chain Reaction (mPCR) assay for simultaneous detection of four important genes associated with the organism E. coli 0157:H7 viz., stx 1, stx 2, eae A, hly A along with an internal amplification control (IAC). The mPCR method developed in the present study is sensitive enough to detect cells as low as 103 CFU ml-1 or g-1 of the food samples. The E. coli 0157:H7 strains having been identified to contain the gene in the mPCR were unequivocally detected positive for the serological and conventional culture method. As E. coli 0157:H7 is qualified as biowarfare agent; this mPCR system is of immense help in detecting them during emergencies of biological war and suspected outbreaks. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://updatepublishing.com/journal/index.php/rrst/article/download/897/881 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
