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Detection and Virulence of Escherichia coli O157:H7 Isolates using Multiplex PCR
| Content Provider | Semantic Scholar |
|---|---|
| Author | Basli, Afrah Abou Alkasim Abou Alkasim Mohammedin, Mohammed Mokhta Farrag, Rasha M. |
| Copyright Year | 2016 |
| Abstract | Abstract Detection of five genes, slt-I, slt-II, eaeA, rfbE, and fliCh7, involved in shiga toxin production was investigated in nine E. coli local isolates using multiplex PCR. Three annealing temperatures were investigated where 53?C was the temperature resulting in the best annealing of the shiga toxin primers in the nine E. coli isolates. A statistical dendrogram was conducted to illustrate the developed gene profiles of the isolates. Slt-II was detected in all E. coli isolates while intimin was the least detected being found in only three isolates; 7, 6, and 8. Multiplex PCR could be used as a valuable diagnostic tool for the detection of shiga toxin-producing E. coli isolates. Also, the current results spot the light on the possible use of multiplex PCR as a virulence measure. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repository.psau.edu.sa/jspui/retrieve/d8809e90-b66a-4aa1-a0bb-75d78974b428/wulfenia%20paper%20(1).pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
