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The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Abdulaev, Najmoutin G. Kakuev, Dmitry L. Tordova, Maria Ridge, Kevin D. Gilliland, Gary L. |
| Copyright Year | 1999 |
| Abstract | The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.ibbr.umd.edu/printpdf/20747 |
| PubMed reference number | 10329774v1 |
| Volume Number | 55 |
| Part | 6 |
| Journal | Acta crystallographica. Section D, Biological crystallography |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Bos taurus Contain (action) Crystal Structure Cyclic GMP Guanosine Diphosphate Nucleoside-Diphosphate Kinase Nucleosides Nucleotides Polypeptides Protein Isoforms Retina monomer nucleoside diphosphate |
| Content Type | Text |
| Resource Type | Article |
