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Real-time bioluminometric method for detection of nucleoside diphosphate kinase activity.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Karamohamed, Samev Nordström, Tomas Nyrén, Pål |
| Copyright Year | 1999 |
| Abstract | A real-time, simple and sensitive method for detection of nucleoside diphosphate (NDP) kinase activity has been developed. The assay is based on detection of ATP, generated in the NDP kinase reaction between a nucleoside triphosphate and adenosine diphosphate (ADP), by the firefly luciferase system. In the presence of 0.3 mM dGTP, the Km for ADP was found to be approximately 30 microM for the NDP kinase from Baker's yeast. In the presence of 250 microM ADP, the Km for dATP alpha S, dTTP alpha S, dGTP, dTTP, dCTP and GTP was found to be approximately 0.01, 0.03, 0.05, 0.25, 0.75 and 0.2 mM, respectively. The assay is sensitive and yields linear responses between 0.05-50 mU. The detection limit was found to be 0.05 mU of NDP kinase. The method was used to detect NDP kinase contamination in commercial enzyme preparations. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.biotechniques.com/multimedia/archive/00007/99264rr02_7858a.pdf |
| PubMed reference number | 10343912v1 |
| Volume Number | 26 |
| Issue Number | 4 |
| Journal | BioTechniques |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 2'-deoxycytidine 5'-triphosphate Adenosine Diphosphate Adenosine Triphosphate Guanosine Triphosphate Killer Factors, Yeast Luciferases Luciferases, Firefly NDP gene Nucleosides deoxyguanosine triphosphate nedaplatin nucleoside diphosphate nucleoside triphosphate nucleoside-diphosphate kinase activity thymidine 5'-triphosphate |
| Content Type | Text |
| Resource Type | Article |
