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NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rosendorff, Adam Sakakibara, Shuhei Lu, Sixin S. Kieff, Elliott Xuan, Yan DiBacco, Alessandra Shi, Yujiang Geno Shi, Yang Gill, Grace B. |
| Copyright Year | 2006 |
| Abstract | Small ubiquitin-like modifier (SUMO) modification of transcription factors is generally associated with repression. Reverse genetic analysis of SUMO-1, and -2 conserved residues emphasized the importance of dual charge reversals in abrogating the critical role of SUMO-2 K33, K35, and K42 in repression. GST-SUMO-2-affinity chromatography followed by liquid chromatography (LC)-MS analysis identified proteins that appeared to bind preferentially to WT SUMO-2 versus SUMO-2 K33E and K35E. LSD1, NXP-2, KIAA0809 (ARIP4), SAE2, RanGAP1, PELP1, and SETDB1 bound to SUMO-2 and not to SUMO-2 K33E, K42E, or K35E and K42E. Although LSD1 is a histone lysine demethylase, and histone H3K4 was demethylated at a SUMO-2-repressed promoter, neither overexpression of a dominant-negative LSD1 nor LSD1 depletion with RNA interference affected SUMO-2-mediated repression, indicating that LSD1 is not essential for repression, in this context. When tethered to a promoter by fusion to Gal4, NXP-2 repressed transcription, consistent with a role for NXP-2 in SUMO-mediated repression. SUMO-2-associated proteins identified in this study may contribute to SUMO-dependent regulation of transcription or other processes. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/103/14/5308.full.pdf |
| PubMed reference number | 16567619v1 |
| Volume Number | 103 |
| Issue Number | 14 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chemical Modifier Dual Histone H3 Lysine 4 Histones KDM1A wt Allele Liquid Chromatography Non-Small Cell Lung Carcinoma PELP1 gene RAD54L2 gene RAN GTPase Activating Protein 1 RNA Interference RNA Processing, Post-Transcriptional Repression, Psychology SETDB1 protein, human SUMO-1 Protein TRANSCRIPTION FACTOR UBA2 gene |
| Content Type | Text |
| Resource Type | Article |
