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Role of substance P (SP) and calcitonin gene-related peptide (CGRP) in gibbon-ape-leukemia virus (GALV) transduction of CD34+ cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shahrokhi, Somayeh Alimoghaddam, Kamran Ghavamzadeh, Ardeshir |
| Copyright Year | 2010 |
| Abstract | AIMS Optimization of transduction condition is an important goal to improve gene transduction. Therefore, we aimed to assess the effect of SP and/or CGRP as novel growth/transducing factors on the efficacy of CD34(+) transduction. METHODS CD34(+) cells were transduced with Gibbon-Ape-Leukemia virus (GALV), containing Neomycin gene. CD34(+) cells were transduced with GALV presence of SP and/or CGRP. Real Time PCR and colony formation assay (CFU-C) was performed. RESULTS Viral vectors titration on Hela cells indicated transduction efficiency of 1×10(6)CFU/ml. Real Time PCR of Neo and CFU-C showed stimulatory role of SP on gene transfer 5.9% and 14.84% compared to 3.6% and 12.58% in control group, while opposite role observed for CGRP 0.89% and 7.86%. Both SP and CGRP showed no significant effect in these assays. DISCUSSION This study showed including of SP in growth factor cocktail is beneficial for CD34(+) transduction, which could be applied to genetic modification procedures. |
| Starting Page | 491 |
| Ending Page | 494 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.npep.2010.08.002 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0143417910000855 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0143417910000855?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 20851465 |
| Alternate Webpage(s) | https://doi.org/10.1016/j.npep.2010.08.002 |
| Journal | Medline |
| Volume Number | 44 |
| Journal | Neuropeptides |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
