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Tissue levels and release of substance P(SP) and calcitonin gene related peptide(CGRP) from nerve fibre sprouts of rat cutaneous nerve neuroma
| Content Provider | Semantic Scholar |
|---|---|
| Author | White, Deborah M. Zimmermann, Manfred Dr |
| Copyright Year | 1987 |
| Abstract | D.M. White and M. Zimmermann. II Physiologisches Institut, Universitiit Heidelberg, Im Neuenheimer Feld 326, D-6900 Heidelberg, F.R.G. Aim: To investigate the changes in tissue levels and release of neuropeptides in neuromas which form following peripheral nerve injury and the influence of morphine on the release. Methods: Neuromas were induced in anaesthetized rats by ligating and cutting the saphenous nerve. Neuropeptide tissue levels were measured in acetic acid extracts of 1, 3 and 5 week (w) old neuromas. The SP tissue levels were also measured after 24hr immersion of neuromas in saline containing morphine (10 pg/ml) in situ. To stimulate a release of the neuropeptides the neuromas were superfused in situ with Tyrode solution containing bradykinin (100 pg/ml). Superfusates and tissue extracts were assayed for SP and CGRP by radioimmunoassay. Results: Levels of the neuropeptides in the 3 w old neuromas were significantly reduced compared to levels in normal saphenous nerve. Levels returned to normal after 5 w. Morphine increases tissue levels of SP in 5 w old neuromas, possibly by inhibiting the basal release of SP. Morphine did not alter the levels of SP in the 3 w old neuroma. Superfusion of neuromas with bradykinin resulted in an increase of SP and CGRP in the superfusate. The amount of SP released following super-fusion with bradykinin varies with the age of the neuroma and is directly proportional to the SP tissue levels. Conclusions: The changes in neuropeptide levels and release may be an important factor in the pathophysiology of neuroma nerve fibre sprouts. (Supported by the DFG.) |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0304-3959(87)91745-3 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0304395987917453 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0304395987917453?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0304-3959%2887%2991745-3 |
| Volume Number | 30 |
| Journal | PAIN |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
