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Analysis of rat insulin II promoter-ghrelin transgenic mice and rat glucagon promoter-ghrelin transgenic mice.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Iwakura, Hiroshi Hosoda, Kiminori Son, Choel Fujikura, Junji Tomita, Tsutomu Noguchi, Michio Ariyasu, Hiroyuki Takaya, Kazuhiko Masuzaki, Hiroaki Ogawa, Yoshihiro Hayashi, Tatsuya Inoue, Gen Akamizu, Takashi Hosoda, Hiroshi Kojima, Masayasu Itoh, Hiroshi Toyokuni, Shinya Kangawa, Kenji Nakao, Kazuwa |
| Copyright Year | 2005 |
| Abstract | We developed and analyzed two types of transgenic mice: rat insulin II promoter-ghrelin transgenic (RIP-G Tg) and rat glucagon promoter-ghrelin transgenic mice (RGP-G Tg). The pancreatic tissue ghrelin concentration measured by C-terminal radioimmunoassay (RIA) and plasma desacyl ghrelin concentration of RIP-G Tg were about 1000 and 3.4 times higher than those of nontransgenic littermates, respectively. The pancreatic tissue n-octanoylated ghrelin concentration measured by N-terminal RIA and plasma n-octanoylated ghrelin concentration of RIP-G Tg were not distinguishable from those of nontransgenic littermates. RIP-G Tg showed suppression of glucose-stimulated insulin secretion. Arginine-stimulated insulin secretion, pancreatic insulin mRNA and peptide levels, beta cell mass, islet architecture, and GLUT2 and PDX-1 immunoreactivity in RIP-G Tg pancreas were not significantly different from those of nontransgenic littermates. Islet batch incubation study did not show suppression of insulin secretion of RIP-G Tg in vitro. The insulin tolerance test showed lower tendency of blood glucose levels in RIP-G Tg. Taking lower tendency of triglyceride level of RIP-G Tg into consideration, these results may indicate that the suppression of insulin secretion is likely due to the effect of desacyl ghrelin on insulin sensitivity. RGP-G Tg, in which the pancreatic tissue ghrelin concentration measured by C-RIA was about 50 times higher than that of nontransgenic littermates, showed no significant changes in insulin secretion, glucose metabolism, islet mass, and islet architecture. The present study raises the possibility that desacyl ghrelin may have influence on glucose metabolism. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/280/15/15247.full.pdf |
| PubMed reference number | 15701644v1 |
| Volume Number | 280 |
| Issue Number | 15 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adrenal cortex Ab:ACnc:Pt:Ser:Qn:RIA Arginine Blood Glucose GCG gene Glucagon In Vitro [Publication Type] One Thousand RIPK1 protein, human Radioimmunoassay Structure of beta Cell of islet Triglycerides ghrelin glucose metabolism insulin secretion insulin, isophane procollagen Type I N-terminal peptide |
| Content Type | Text |
| Resource Type | Article |
