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Differential monocyte adhesion and adhesion molecule expression in venous and arterial endothelial cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kalogeris, Theodore J. Kevil, Christopher G. Laroux, F. Stephen Coe, Laura L. Phifer, Travis J. Alexander, J. S. |
| Copyright Year | 1999 |
| Abstract | We compared U-937 cell adhesion and adhesion molecule expression in human umbilical venous (HUVECs) and arterial (HUAECs) endothelial cells exposed to tumor necrosis factor (TNF), interleukin-1, and lipopolysaccharide (LPS). TNF and LPS stimulated vascular cell adhesion molecule (VCAM)-1 surface expression and adhesion of U-937 monocyte-like cells to HUVECs but not to HUAECs. Antibody studies demonstrated that in HUVECs at least 75% of the adhesion response is VCAM-1 mediated. Interleukin-1 stimulated U-937 cell adhesion to and VCAM-1 surface expression in both HUVECs and HUAECs. Pyrrolidinedithiocarbamate and the proteasome inhibitor MG-132 blocked TNF- and LPS-stimulated U-937 cell adhesion to HUVECs. These agents also significantly decreased TNF- and LPS-stimulated increases in HUVEC surface VCAM-1. TNF increased VCAM-1 protein and mRNA in HUVECs that was blocked by pyrrolidinedithiocarbamate. However, neither TNF or LPS stimulated VCAM-1 expression in HUAECs. TNF stimulated expression of both intercellular adhesion molecule-1 and E-selectin in HUVECs, but in HUAECs, only intercellular adhesion molecule-1 was increased. Electrophoretic mobility shift assays demonstrated no difference in the pattern of TNF-stimulated nuclear factor-κB activation between HUVECs and HUAECs. These studies demonstrate a novel and striking insensitivity of arterial endothelium to the effects of TNF and LPS and indicate a dissociation between the ability of HUAECs to upregulate nuclear factor-κB and VCAM-1. |
| Starting Page | L9 |
| Ending Page | L19 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/276/1/l9.full.pdf |
| PubMed reference number | 29591972v1 |
| Alternate Webpage(s) | https://doi.org/10.1152/ajplung.1999.276.1.L9 |
| DOI | 10.1152/ajplung.1999.276.1.l9 |
| Journal | American journal of physiology. Lung cellular and molecular physiology |
| Volume Number | 276 |
| Issue Number | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Adhesion Molecules E-Selectin Electrophoretic Mobility Shift Assay Endothelium of artery Intercellular adhesion molecule 1 Leukemia, B-Cell Lipopolysaccharides MG 132 Neoplasms Pyrrolidinedithiocarbamate Selectins Tumor Necrosis Factors Umbilicus (Anatomy) Vascular Cell Adhesion Molecule-1 |
| Content Type | Text |
| Resource Type | Article |
