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High-resolution melting-based quantitative analysis of RASSF1 methylation in breast cancer.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Stuopelytė, Kristina Daniūnaitė, Kristina Laurinavičienė, Aida Ostapenko, Valerijus Jarmalaitė, Sonata |
| Copyright Year | 2013 |
| Abstract | BACKGROUND AND OBJECTIVE Breast cancer is the leading cause of death from cancer among women worldwide. The aberrant promoter methylation of tumor suppressor genes is a typical epigenetic alteration for breast cancer and can be detected in early carcinogenesis. High-throughput and cost-effective methods are needed for the early and sensitive detection of epigenetic changes in clinical material. The main purpose of our study was to optimize a high-resolution melting (HRM) assay for the reliable and quantitative assessment of RASSF1 gene methylation, which is considered one of the earliest epigenetic alterations in breast cancer. MATERIAL AND METHODS A total of 76 breast carcinomas and 10 noncancerous breast tissues were studied by means of HRM and compared with the results obtained by means of quantitative methylation-specific polymerase chain reaction (QMSP) and methylation-specific polymerase chain reaction (MSP). RESULTS Both quantitative methods, HRM and QMSP, showed a similar specificity and sensitivity for the detection of RASSF1 methylation in breast cancer (about 80% and 70%, respectively). In breast cancer, the mean methylation intensity of RASSF1 was 42.5% and 48.6% according to HRM and QMSP, respectively. Both methods detected low levels of methylation (less than 5%) in noncancerous breast tissues. In comparison with quantitative methods, MSP showed a lower sensitivity (70%), but a higher specificity (80%) for the detection of RASSF1 methylation in breast cancer. CONCLUSIONS HRM is as a simple, cost-effective method for the reliable high-throughput quantification of DNA methylation in clinical material. |
| Starting Page | 78 |
| Ending Page | 83 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://res.mdpi.com/def502002fa05fe1cf58c3840e846e1b0fc1d93f2fe8043c21cd2f0166cbb430371a9d4c8613c663f3f93edca60f664689000f6b4d9e38ffe61abc2eb91f4f971be9348453281e86371dace80565db4c9c1d2d2cf92f1ef10424ecbf9e39a043ce4398b55175c2ca22619ea3796131186faa833ecffa93b3c136d14aea1ea7262e8562b1cd930ed866f5b147f2e73e2b45f8a0?attachment=1&filename= |
| PubMed reference number | 23888343v1 |
| Volume Number | 49 |
| Issue Number | 2 |
| Journal | Medicina |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Body tissue Breast Carcinoma Carcinogenesis Cessation of life Genes, Suppressor Less Than Mammary Neoplasms Methylation Nephroblastoma Polymerase Chain Reaction Quantitation RASSF1 gene Tumor Suppressor Genes study of epigenetics |
| Content Type | Text |
| Resource Type | Article |
