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DFNA5 promoter methylation a marker for breast tumorigenesis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Croes, Lieselot Beeck, Ken Op de Pauwels, Patrick Berghe, Wim Vanden Peeters, Marc Fransén, Erik Camp, Guy Van |
| Copyright Year | 2017 |
| Abstract | BACKGROUND Identification of methylation markers that are sensitive and specific for breast cancer may improve early detection. We hypothesize that DFNA5 promoter methylation can be a valuable epigenetic biomarker, based upon strong indications for its role as tumor suppressor gene and its function in regulated cell death. RESULTS Statistically different levels of methylation were seen, with always very low levels in healthy breast reduction samples, very high levels in part of the adenocarcinoma samples and slightly increased levels in part of the normal tissue samples adjacent the tumor. One of the CpGs (CpG4) showed the best differentiation. A ROC curve for DFNA5 CpG4 methylation showed a sensitivity of 61.8% for the detection of breast cancer with a specificity of 100%. MATERIALS AND METHODS We performed methylation analysis on four CpGs in the DFNA5 promoter region by bisulfite pyrosequencing on 123 primary breast adenocarcinomas and 24 healthy breast reductions. For 16 primary tumors, corresponding histological normal tissue adjacent to the tumor was available. CONCLUSIONS We conclude that DFNA5 methylation shows strong potential as a biomarker for detection of breast cancer. Slightly increased methylation in histologically normal breast tissue surrounding the tumor suggests that it may be a good early detection marker. |
| Starting Page | 31948 |
| Ending Page | 31958 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.oncotarget.com/index.php?journal=oncotarget&op=download&page=article&path%5B%5D=16654&path%5B%5D=53259 |
| PubMed reference number | 28404884 |
| Alternate Webpage(s) | https://doi.org/10.18632/oncotarget.16654 |
| DOI | 10.18632/oncotarget.16654 |
| Journal | Oncotarget |
| Volume Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adenocarcinoma Biological Markers Body tissue Carcinogenesis Cell Death Cessation of life DEAFNESS, AUTOSOMAL DOMINANT 5 (disorder) DFNA5 gene Detection Early Diagnosis Mammary Gland Parenchyma Mammary Neoplasms Methylation Promoter Regions, Genetic Pyrosequencing Reduction mammaplasty Tumor Suppressor Genes hydrogen sulfite primary tumor study of epigenetics |
| Content Type | Text |
| Resource Type | Article |
