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Detection of Peach latent mosaic viroid by PCR
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ryšánek, Pavel Zouhar, Miloslav Hassan, Mohamed Khalifa |
| Copyright Year | 2017 |
| Abstract | Peach latent mosaic viroid (PLMVd) is widespread in peach all over the world. It has never been reported from the Czech Republic. That is why we adapted specific and sensitive method for its detection, PCR, to be able to prove its possible occurrence and for certification purposes. Primers PLMVdR, PLMVdF1 and PLMVdF2 were designed on the basis of published RNA sequences. Products of amplification are 208 and 114 bp long for PLMVdF1 and PLMVdF2, respectively. Four PLMVd isolates from Dr Di Serio (CNR Bari) were used as standards. Potato spindle tuber viroid and Hop latent viroid infected plant material and also healthy material were used to check detection specifity. Both RNA extraction from plant material and PCR were optimalized so that this method of PLMVd detection can also be used for certification purposes. |
| Starting Page | 249 |
| Ending Page | 251 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| DOI | 10.17221/10458-pps |
| Volume Number | 38 |
| Alternate Webpage(s) | https://www.agriculturejournals.cz/publicFiles/235426.pdf |
| Alternate Webpage(s) | https://doi.org/10.17221/10458-pps |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
