NDLI: Development of a Rapid RT-PCR Test for the Detection of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid and Apple Scar Skin Viroid in Fruit Trees from Tunisia

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Development of a Rapid RT-PCR Test for the Detection of Peach Latent Mosaic Viroid, Pear Blister Canker Viroid, Hop Stunt Viroid and Apple Scar Skin Viroid in Fruit Trees from Tunisia

Content Provider	Semantic Scholar
Author	Hassen, I. Fekih Roussel, Sabine Kummert, Jean Fakhfakh, Hatem Marrakchi, Mohamed Jijakli, Mohamed Haïssam
Copyright Year	2006
Abstract	A reverse transcription polymerase chain reaction was developed to investigate the occurrence ofpeach latent mosaic viroid� (PLMVd), � pear blister canker viroid� (PBCVd), � hop stunt viroid� (HSVd) andapple scar skin viroid� (ASSVd) on fruit trees (peach, pear, almond and apple) in Tunisia. The test was initially performed with total RNA preparations from selected isolates and then applied to total RNA preparations from leaf or bark tissues of fruit trees collected in the north of Tunisia and the Sahel. PLMVd was found to occur in peach and pear trees, HSVd in pear, peach and almond trees, and PBCVd in pear trees. Mixed PBCVd-HSVd and PLMVd-HSVd infections occurred naturally in pear trees. ASSVd was not detected in any samples from apple trees. The identity of the detected viroids was confirmed by comparing their sequences with those of other previously characterized isolates. The test was then simplified by direct use of diluted crude plant extracts. The results obtained from crude sap extracts of leaves or bark tissues and from total RNA preparations were identical. This improved test is thus quick and useful for large-scale routine analy- sis. It can be used in a certification programme to con- tribute to prevention of the occurrence and spread of PLMVd, HSVd, PBCVd and ASSVd in Tunisia.
Starting Page	217
Ending Page	223
Page Count	7
File Format	PDF HTM / HTML
DOI	10.1111/j.1439-0434.2006.01087.x
Volume Number	154
Alternate Webpage(s)	https://orbi.uliege.be/bitstream/2268/36866/1/N%C2%B0256-Fekih%20Hassen%20I%20%202006.pdf
Alternate Webpage(s)	https://doi.org/10.1111/j.1439-0434.2006.01087.x
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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