NDLI: Expression of hepatitis B virus genes in early embryonic cells originated from hamster ova and human spermatozoa transfected with the complete viral genome.

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Expression of hepatitis B virus genes in early embryonic cells originated from hamster ova and human spermatozoa transfected with the complete viral genome.

Content Provider	Semantic Scholar
Author	Ali, Bahy Ahmed Huang, Tian-Hua Salem, Halima-Hassan Xie, Qing-Dong
Copyright Year	2006
Abstract	AIM To detect the expression of hepatitis B virus (HBV) genes (HB S and C genes) in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fertilization (IVF) technique. METHODS Human sperm-mediated HBV genes were delivered into zona-free hamster oocytes by the IVF method. Polymerase chain reaction (PCR) was used to detect HB S and pre-Core/Core (pre-C/C) coding genes both in one- and two-cell embryos. Reverse transcription-PCR (RT-PCR) analysis was used to study the expression of the two genes. Fluorescence in situ hybridization (FISH) analysis using the full-length HBV DNA as the hybridization probe was performed to confirm the integration of viral DNA in the host embryonic genome. RESULTS Both HB S and pre-C/C coding genes are present and transcribed in one- and two-cell embryos originated from hamster ova IVF with human spermatozoa carrying HBV DNA sequences. CONCLUSION Sperm-mediated HBV genes are able to replicate and express themselves in early embryonic cells. These results provide direct evidence that HBV DNA could transmit vertically to the next generation via the male germ line.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.asiaandro.com/news/upload/20130912-aja200653a.pdf
PubMed reference number	16625276v1
Volume Number	8
Issue Number	3
Journal	Asian journal of andrology
Language	English
Access Restriction	Open
Subject Keyword	Embryo Embryonic Cell Fertilization in Vitro Fluorescent in Situ Hybridization Hepatitis B Virus Hepatitis C virus Herpes zoster disease Herpesvirus 1, Cercopithecine Nucleic Acid Hybridization Ovum Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Spermatozoa Cell Count viral capsid secondary envelopment viral genome location
Content Type	Text
Resource Type	Article

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