NDLI: Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis.

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Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis.

Content Provider	Semantic Scholar
Author	Eden-Mccutchan, F. Vodkin, Michael Leppla, Stephen H.
Copyright Year	1988
Abstract	The nucleotide sequence of the protective antigen (PA) gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined. PA is one of three proteins comprising anthrax toxin; and its nucleotide sequence is the first to be reported from B. anthracis. The open reading frame (ORF) is 2319 bp long, of which 2205 bp encode the 735 amino acids of the secreted protein. This region is preceded by 29 codons, which appear to encode a signal peptide having characteristics in common with those of other secreted proteins. A consensus TATAAT sequence was located at the putative -10 promoter site. A Shine-Dalgarno site similar to that found in genes of other Bacillus sp. was located 7 bp upstream from the ATG start codon. The codon usage for the PA gene reflected its high A + T (69%) base composition and differed from those of genes for bacterial proteins from most other sequences examined. The TAA translation stop codon was followed by an inverted repeat forming a potential termination signal. In addition, a 192-codon ORF of unknown significance, theoretically encoding a 21.6-kDa protein, preceded the 5' end of the PA gene.
Starting Page	287
Ending Page	300
Page Count	14
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.dtic.mil/dtic/tr/fulltext/u2/a204674.pdf
PubMed reference number	3148491v1
Volume Number	69
Issue Number	2
Journal	Gene
Language	English
Access Restriction	Open
Subject Keyword	3' Flanking Region Amino Acids Aortic Aneurysm, Thoracic Bacillus anthracis Bacillus species Base Composition Base Sequence Cell secretion Codon (nucleotide sequence) Codon, Initiator Codon, Terminator Encode (action) Flank (surface region) Mucin-1 Antigen Nucleotides Open Reading Frames Reading Frames (Nucleotide Sequence) Signal Peptides anthrax toxin
Content Type	Text
Resource Type	Article

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