Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions.

Content Provider	Semantic Scholar
Author	Shiroza, T. Kuramitsu, Howard K.
Copyright Year	1988
Abstract	The nucleotide sequence of the ftf gene from Streptococcus mutants GS-5 was determined. The deduced amino acid sequence indicates that the unprocessed fructosyltransferase gene product has a molecular weight of 87,600. A typical streptococcal signal sequence is present at the amino terminus of the protein. The processed enzyme is relatively hydrophilic and has a pI of 5.66. An inverted repeat structure was detected upstream from the ftf gene and may function in the regulation of fructosyltransferase expression. Sequencing of the regions flanking the gene revealed the presence of four other putative open reading frames (ORFs). Two of these, ORFs 2 and 3, appear to code for low-molecular-weight proteins containing amino acid sequences sharing homology with several gram-positive bacterial DNA-binding proteins. In addition, ORF 3 is transcribed from the ftf DNA coding strand. Partial sequencing of ORF 4 suggests that its gene product may be an extracellular protein.
Starting Page	1381
Ending Page	1386
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jb.asm.org/content/170/2/810.full.pdf
PubMed reference number	2828325v1
Volume Number	170
Issue Number	2
Journal	Journal of bacteriology
Language	English
Access Restriction	Open
Subject Keyword	Amino Acid Sequence Amino Acids Base Sequence Flank (surface region) Frame (physical object) Genome Encoded Entity Homologous Gene Molecular Weight Nucleotides Open Reading Frames Proteins Reading Frames (Nucleotide Sequence) Sequence Analysis Signal Peptides Streptococcus mutans gram
Content Type	Text
Resource Type	Article