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tection of telomerase activity in a single cell using stem-loop primer-mediated exponential ampli fi cation ( SPEA ) with near zero nonspeci fi c signal †
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Honghong Wang, Hui Liu, Chenghui Duan, Xinrui Li, Zhengping |
| Copyright Year | 2016 |
| Abstract | Telomerase is a crucial biomarker for cancers. Its reliable and sensitive detection, particularly in a single cell, has great significance for the early diagnosis of cancers, studies on tumor progression and anticancer therapy, which remain a challenge, due to nonspecific amplification. Herein, we developed a novel stem-loop primer-mediated exponential amplification (SPEA) strategy, which can specifically and efficiently amplify the telomerase-elongated telomere repeat unit with near zero nonspecific signal. The SPEA-based assay can accurately detect telomerase activity in the crude lysate of a single cell and is suited for detecting the cellular heterogeneity arising from cell-to-cell variations. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://pubs.rsc.org/en/content/articlepdf/2016/sc/c6sc00802j |
| Alternate Webpage(s) | https://pubs.rsc.org/en/content/articlepdf/2016/sc/c6sc00802j |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
