screening test of Listeria monocytogenes in food samples using a real-time loop-mediated isothermal ampli fi cation turbidity assay

Content Provider	Semantic Scholar
Author	Wachiralurpan, Sirirat Sriyapai, Thayat Areekit, Supatra Sriyapai, Pichapak Thongphueak, Dueantem Santiwatanakule, Somchai Chansiri, Kosum
Copyright Year	2017
Abstract	A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no crossreactions. The limits of detection for purified genomic DNA and pure culture were 800 pg mL 1 and 2.82 10 CFU mL , respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products.
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Alternate Webpage(s)	https://pubs.rsc.org/en/content/articlepdf/2017/ay/c7ay01750b
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article