NDLI: Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro

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Histone deacetylase inhibitor, valproic acid, radiosensitizes the C6 glioma cell line in vitro

Content Provider	Semantic Scholar
Author	Zhou, Yong Xu, Ying Wang, Han Niu, Junjie Hou, Huaying Jiang, Yuhua
Copyright Year	2014
Abstract	Valproic acid (VPA) is a well-tolerated drug that is used to treat seizure disorders and that has recently been shown to inhibit histone deacetylase. The present study investigated the effects of VPA on the radiosensitization of the rat C6 glioma cell line in vitro. To select an appropriate treatment concentration and time, MTT and flow cytometry assays were performed to measure the inhibitory effects of VPA at various concentrations and incubation time-points. The radiosensitizing effect of VPA was determined using clonogenic experiments. VPA- and radiation-induced C6 apoptosis was analyzed using quantitative polymerase chain reaction and western blot analysis. Cell proliferation was significantly inhibited by VPA in a time- and dose-dependent manner (P<0.05). VPA enhanced radiation-induced C6 cell death and there was clear inhibition of clonogenic formation [sensitizer enhancement ratio (SER), 1.30]. This effect was closely associated with the concentration of VPA. VPA treatment decreased the mRNA and protein levels of Bcl-2, whereas increased changes were detected with Bax. At a concentration of 0.5 mmol/l, VPA had a low toxicity and enhanced the radiosensitization of the C6 cells. VPA may radiosensitize glioma cells by inhibiting cellular proliferation and inducing apoptosis by regulating apoptosis-related molecular changes.
Starting Page	203
Ending Page	208
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/ol/7/1/203/download
PubMed reference number	24348849v1
Volume Number	7
Journal	Oncology letters
Language	English
Access Restriction	Open
Subject Keyword	Adverse reaction to drug Apoptosis Bax protein Cell Death Cell Proliferation Cessation of life Epilepsy Flow Cytometry Glioma Histone Deacetylase Histones Inhibition Polymerase Chain Reaction Seizures Valproic Acid Western Blotting monooxyethylene trimethylolpropane tristearate radiosensitization
Content Type	Text
Resource Type	Article

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