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A simple wax-embedding method for isolation of aphid hemolymph for detection of luteoviruses in the hemocoel.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Sijun Bonning, Bryony C. Miller, W. Allen |
| Copyright Year | 2006 |
| Abstract | A protocol for isolating hemolymph from viruliferous aphids has been developed. This method uses warm melted wax to immobilize the aphid. Following removal of a hind leg, the hemolymph can be collected readily. Flushing with RNase-free water allows for collection of sufficient hemolymph for RNA extraction from individual aphids. The extracted RNA was successfully used for detection of barley yellow dwarf virus (BYDV) and pea enation mosaic virus (PEMV) from individual viruliferous Rhopalosiphum padi and Acyrthosiphon pisum aphids, respectively. A TaqMan real-time RT-PCR protocol for quantitation of PEMV in the hemolymph of individual aphids was developed. The wax-embedding hemolymph collection technique provides a useful tool for studying molecular interactions between persistent and circulative plant viruses and their insect vectors. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.public.iastate.edu/~wamiller/dir2/LiuWaxEmbedAphidJVM06.pdf |
| PubMed reference number | 16307802v1 |
| Volume Number | 132 |
| Issue Number | 1-2 |
| Journal | Journal of virological methods |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aphids Aphis chenopodii glauci preparation Barley yellow dwarf virus Dwarfism Embedding Extraction Flushing ISOLATE COMPOUND Immobilization Luteovirus Mosaic Organism Mosaic Viruses Pea enation mosaic virus-1 Plant Viruses Quantitation RNA Waxes Yellow Fever Vaccine |
| Content Type | Text |
| Resource Type | Article |
