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The nucleotide sequence and luteovirus-like nature of RNA 1 of an aphid non-transmissible strain of pea enation mosaic virus.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Demler, S. A. Zoeten, G. A. De |
| Copyright Year | 1991 |
| Abstract | An examination of the genomic strategy of pea enation mosaic virus (PEMV) RNA 1 has verified strong organizational and sequence relationships between PEMV and the beet western yellows-potato leafroll luteovirus subgroup. Sequence analysis of RNA 1 demonstrated five predominant open reading frames (ORFs). The extreme 5' ORF encodes a 34K product of unknown function. The second ORF encodes an 84K product which overlaps 90% of ORF 1 (in a unique reading frame) and is expressed by internal initiation beginning at the second start codon from the 5' terminus. This protein contains a protease-like motif characteristic of serine- and cysteine-based proteases, suggesting involvement in post-translational processing of viral translation products. The third ORF is characterized by a number of RNA polymerase motifs and a helicase-like motif typical of RNA-dependent RNA polymerases. It overlaps (out of frame) the ORF 2 product and is proposed to be expressed by a frameshift fusion of the ORF 2 and ORF 3 products. The fourth ORF encodes the viral coat protein, and is immediately followed in frame by a 33K ORF thought to represent the aphid transmission subunit of the PEMV virion. Northern blot analysis of polysome-associated RNA suggests that both products are expressed from an 1800 nucleotide subgenomic mRNA, with the 33K product expressed as a read-through fusion with the coat protein monomer. |
| File Format | PDF HTM / HTML |
| DOI | 10.1099/0022-1317-72-8-1819 |
| PubMed reference number | 1875194 |
| Journal | Medline |
| Volume Number | 72 |
| Part | 8 |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/72/8/JV0720081819.pdf?accname=guest&checksum=0433B22723C9816ECC0FDF85F6DCDC7F&expires=1543614326&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/72/8/JV0720081819.pdf?accname=guest&checksum=AC7B7A44DA5304968C84E6BFB75D0251&expires=1544092064&id=id |
| Alternate Webpage(s) | https://www.microbiologyresearch.org/docserver/fulltext/jgv/72/8/JV0720081819.pdf?accname=guest&checksum=36F202E34721A962F53A9D996471EA44&expires=1546708585&id=id |
| Alternate Webpage(s) | https://doi.org/10.1099/0022-1317-72-8-1819 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
