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Emulsified Isoflurane Preconditioning Reduces Lung Injury Induced By Hepatic Ischemia/Reperfusion in Rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Zhen-Meng Huang, Sheng-Dong Song, Shao-Hua Wu, Fei-Xiang Yu, Wei-Feng |
| Copyright Year | 2011 |
| Abstract | OBJECTIVE To investigate whether emulsified isoflurane preconditioning could reduce lung injury induced by hepatic I/R in rats and its mechanism. MATERIALS AND METHODS 32 pentobarbital-anesthetized Sprague-Dawley rats were equally randomized into four groups: laparotomy group (Sham group), hepatic I/R and normal saline infusion group (I/R+S group), I/R and lipid vehicle infusion (I/R+V group), or I/R and 8% emulsified isoflurane infusion (I/R+E group) at the rate of 8 ml·kg(-1)·h(-1) for 30 min. Blood supply of the hepatic artery and portal vein to the left and the median liver lobes was occluded for 90 min after 30-min washout time. Reperfusion was allowed to proceed for 4 h before sacrifice of the animals. Lung injury was observed histologically. Neutrophil infiltration and TNF-α concentration in serum and lung were measured. Changes of wet-to-dry weight ratios in lung tissue, ICAM-1 expression and NF-κB activity in lung after hepatic I/R were determined. RESULTS Compared with I/R+S or I/R+V group, emulsified isoflurane preconditioning reduced hepatic I/R-induced lung histologic injury and inhibited the increase of myeloperoxidase (MPO) activity in the lung tissue markedly (5.5±1.37 and 5.22±1.33 vs 3.81±1.62 U/g, P<0.05). In addition, both serum and lung tissue TNF-α levels were reduced in I/R+E group (104.58±31.40 and 94.60±22.23 vs 72.44±17.28 pg/ml, P<0.05; 393.51±88.22 and 405.46±102.87 vs 292.62±74.56 pg/ml, P<0.01). Emulsified isoflurane preconditioning also inhibited the increase of ICAM-1 expression (0.79±0.17 and 0.84±0.24 vs 0.62±0.21, P<0.05) and NF-κB translocation (4.93±0.48 and 4.76±0.57 vs 4.01±0.86, P<0.05) in the lung tissue markedly. CONCLUSIONS Emulsified isoflurane preconditioning markedly attenuated hepatic I/R-induced lung injury in rats, which may be hopefully applied to the clinical treatment of organ injury caused by hepatic surgery, transplantation or hemorrhagic shock. |
| Starting Page | 353 |
| Ending Page | 361 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.7150/ijms.8.353 |
| PubMed reference number | 21698053 |
| Journal | Medline |
| Volume Number | 8 |
| Alternate Webpage(s) | http://www.medsci.org/v08p0353.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/d2/86/ijmsv08p0353.PMC3119377.pdf |
| Alternate Webpage(s) | https://doi.org/10.7150/ijms.8.353 |
| Journal | International journal of medical sciences |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
