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Trypanosoma cruzi: phosphatidylinositol 3-kinase and protein kinase B activation is associated with parasite invasion.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wilkowsky, Silvina Elizabeth Barbieri, M. A. |
| Copyright Year | 2001 |
| Abstract | Multiple signal transduction events are triggered in the host cell during invasion by the protozoan parasite Trypanosoma cruzi. Here, we report the regulation of host cell phosphatydilinositol 3-kinase (PI3K) and protein kinase B (PKB/Akt) activities by T. cruzi during parasite-host cell interaction. Treatment of nonphagocytic cells (Vero, L(6)E(9), and NIH 3T3) and phagocytic cells (human and J774 murine macrophages) with the selective PI3K inhibitors Wortmannin and LY294002 significantly impaired parasite invasion in a dose-dependent fashion. A strong activation of PI3K and PKB/Akt activities in Vero cells was detected when these cells were incubated with trypomastigotes or their isolated membranes. Consistently, we were unable to detect activation of PI3K or PKB/Akt activities in host cells during epimastigote (noninfective) membrane-host cell interaction. Infection of transiently transfected cells containing an inactive mutant PKB showed a significant inhibition of invasion compared with the active mutant-transfected cells. T. cruzi PI3K-like activity was also required in host cell invasion since treatment of trypomastigotes with PI3K inhibitors prior to infection reduced parasite entry. Taken together, these results indicate that PI3K and PKB/Akt activation in parasites, as in host cells induced by T. cruzi, is an early invasion signal required for successful trypomastigote internalization. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cmdr.ubc.ca/trainingprogram/papers/jp_May%2020b_05.pdf |
| PubMed reference number | 11262178v1 |
| Volume Number | 264 |
| Issue Number | 2 |
| Journal | Experimental cell research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 1-Phosphatidylinositol 3-Kinase Inactive - Biochemical Activity Level LY 294002 Parasite Encystment Parasites Phagocytes Protein Kinases Proto-Oncogene Proteins c-akt Protozoa RAC-Alpha Serine/Threonine Kinase Signal Transduction Tissue membrane Trypanosoma cruzi Ab.IgM:Titr:Pt:Ser:Qn entry into host cell incubated phosphatidylinositol 3-kinase complex, class IA wortmannin |
| Content Type | Text |
| Resource Type | Article |
