NDLI: Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes.

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Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes.

Content Provider	Semantic Scholar
Author	Pauliukaitė, Rasa Schoenleber, Monika M. Vadgama, Pankaj Brett, Christopher M. A.
Copyright Year	2008
Abstract	Protective polymer coatings have been used to enhance the retention of enzymes in sol-gel films as immobilisation phases in electrochemical biosensors. Carbon film electrodes were electrochemically modified with poly(neutral red) (PNR). These electrodes were coated with oxysilane sol-gels incorporating glucose oxidase and an outer coating of carboxylated PVC (CPVC) or polyurethane (PU), with and without Aliquat-336 or isopropyl myristate (IPM) plasticizer, was applied. The biosensors were characterised electrochemically using cyclic voltammetry and amperometry, electrochemical impedance spectroscopy and scanning electron microscopy. Impedance spectra showed that the electrode surface is most active when the sol-gel-GOx layer is not covered with a membrane. However, membranes without plasticizer extend the lifetime of the biosensor to more than 2 months when PU is used as an outer membrane. The linear range of the biosensors was found to be 0.05-0.50 mM of glucose and the biosensor with PU outer membrane exhibited higher sensitivity (ca.117 nA mM(-1)) in the region of linear response than that with CPVC. The biosensors were applied to glucose measurement in natural samples of commercial orange juice.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://estudogeral.sib.uc.pt/bitstream/10316/7774/1/obra.pdf
PubMed reference number	18080811v1
Volume Number	390
Issue Number	4
Journal	Analytical and bioanalytical chemistry
Language	English
Access Restriction	Open
Subject Keyword	Biological Factors Biosensors Carbon Coating Excipient Dielectric Spectroscopy Erythrocytes Ethanol 0.62 ML/ML Topical Gel GLUCOSE OXIDASE Glucose Metabolism Disorders Greater Than HAO1 gene Immobilization Plasticizers Polymers Polyurethanes Polyvinyl Chloride Quantitative impedance Scanning Electron Microscopy Sodium Solution Dosage Form Tissue membrane anatomical layer carboxylate electrode isopropyl myristate outer membrane oxidation
Content Type	Text
Resource Type	Article

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