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Glucose sensor for flow injection analysis of serum glucose based on immobilization of glucose oxidase in titania sol-gel membrane.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yu, Jiuhong Liu, Songqin Ju, Huangxian |
| Copyright Year | 2003 |
| Abstract | A novel amperometric glucose sensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared with a vapor deposition method. The sol-gel film was uniform, porous and showed a very low mass transport barrier and a regular dense distribution of GOD. Titania sol-gel matrix retained the native structure and activity of entrapped enzyme and prevented the cracking of conventional sol-gel glasses and the leaking of enzyme out of the film. With ferrocenium as a mediator the glucose sensor exhibited a fast response, a wide linear range from 0.07 to 15 mM. It showed a good accuracy and high sensitivity as 7.2 microA cm(-2) mM(-1). The general interferences coexisted in blood except ascorbic acid did not affect glucose determination, and coating Nafion film on the sol-gel film could eliminate the interference from ascorbic acid. The serum glucose determination results obtained with a flow injection analysis (FIA) system showed an acceptable accuracy, a good reproducibility and stability and indicated the sensor could be used in FIA determination of glucose. The vapor deposition method could fabricate glucose sensor in batches with a very small amount of enzyme. |
| Starting Page | 13 |
| Ending Page | 18 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://sklac.nju.edu.cn/hxju/lunwenlunzhu/paper2001-2003/119%20BB%20Yu%20JH.pdf |
| PubMed reference number | 14615099v1 |
| Volume Number | 19 |
| Issue Number | 4 |
| Journal | Biosensors & bioelectronics |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affinity Ascorbic Acid Coating Excipient Exhibits as Topic Flow Injection Analysis GLUCOSE OXIDASE Glucose measurement Mast/Stem Cell Growth Factor Receptor Kit, human Mediator brand of benfluorex hydrochloride Nafion Natural Science Disciplines Tissue membrane serum glucose vapor |
| Content Type | Text |
| Resource Type | Article |
