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Longitudinal study of T cell receptor usage to myelin basic protein in multiple sclerosis patients
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lovett, Amy E. Martin, Roland McFarland, Henry F. Utz, Ursula |
| Copyright Year | 1994 |
| Abstract | lutroduetlon: [s order to investigate the role of macropheges (M@) in the processes of myelination, demyelination and remyclination, the M@ component of aggre~ng rat bre/n celt cultures has been supplereented with peritoneal M#. Materials m~l Methods: M# were added to a suspension of dissociated rat fetal t e l e ~ o n cellsin serum-free medium at either 5% or 10% of the te!encephalon cell number. Cultures were treated with either demyelinating antibody [am yelin/oligodendrecyte 8lycoprotein (a-MOG) with complement], intederon-y (IFN-~t) or intedeukinlu ([L1o¢), Following removal of these agents, cultures were maintained until day 40. Control and M~-mriched aggregates were sampled at intervals throughout the culture period. Myelin basic protein (MBP) content, an indicator of the extent of reyelieafion, was determined and Northern blotting and slot blot techniques were used to analyse reRNAs encoding myelin proteins, Re=olts: Qualitative irereunocytochemistry dereoustrates that M~p, added to ihe cell suspensions at the start of the culture period, successfully integrate into aggregates. M4,-endched cultures show increased reyelination. The degree of demyelination in cultures treated with IFN-y was similar in M~-enriched and in control cultures however dereyelination induced by ct-MOG was more extensive in the former. Both normal and M~-emiched cultm'es show increased MBP content on removal of ILlu or Ifn-y which reirrors original myetination. Conclus ions : The presence of M4t in increased numbers enhances both reyelination in ag8regate cultures and rereyelination following cytokine-induced dereyelination in this system. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0165-5728(94)90417-0 |
| Volume Number | 54 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0165572894904170 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0165572894904170?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0165-5728%2894%2990417-0 |
| Journal | Journal of Neuroimmunology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
