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Cornin protects SH-SY5Y cells against oxygen and glucose deprivation-induced autophagy through the PI3K/Akt/mTOR pathway
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ding, Changling Zhang, Jie Ming Li, Baoyuan Ding, Zhaoxing Cheng, Wenna Gao, Fei Zhang, Ye Xu, Yangyang Zhang, Shuping |
| Copyright Year | 2017 |
| Abstract | It has been reported that cornin may reduce neuronal death during cerebral ischemia; however, little is known about the molecular mechanism of the role of corninin autophagy in SH‑SY5Y neuronal cells. In the present study, oxygen‑glucose deprivation (OGD)‑treated cells were used as a cerebral ischemia model in vitro. The results demonstrated that cornin was able to reduce neuronal cell loss, increase the apoptosis regulator Bcl‑2/apoptosis regulator BAX ratio, and decrease the protein levels of caspase‑3. In addition, cornin decreased the microtubule‑associated proteins 1A/1B light chain 3B (LC3)‑II/LC3‑I ratio and beclin‑1 protein expression, and resulted in an upregulation in phosphorylated (p)‑RAC‑α serine/threonine‑protein kinase (Akt), p‑protein kinase mTOR (mTOR) in OGD‑treated SH‑SY5Y cells. Additionally, it was observed that following inhibition of PI3K/Akt by LY294002, the levels of p‑Akt and p‑mTOR were markedly decreased, and the LC3‑II/LC3‑I ratio and beclin‑1 were increased. Similarly, following inhibition of mTOR by rapamycin, LC3‑II/LC3‑I and Beclin‑1 were significantly increased in SH‑SY5Y cells. These results indicated that cornin protected SH‑SY5Y cells against OGD‑induced autophagy through the PI3K/Akt/mTOR pathway. |
| Starting Page | 87 |
| Ending Page | 92 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://spandidos-publications.com/mmr/17/1/87/download |
| PubMed reference number | 29115424v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/mmr.2017.7864 |
| DOI | 10.3892/mmr.2017.7864 |
| Journal | Molecular medicine reports |
| Volume Number | 17 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Regulatory Proteins Autophagy Bax protein Cerebral Ischemia FRAP1 protein, human Glucose Immunoglobulin lambda-Chains LY 294002 Nerve Degeneration Oxygen Proto-Oncogene Proteins c-akt REM Sleep Deprivation Sirolimus Up-Regulation (Physiology) protein expression |
| Content Type | Text |
| Resource Type | Article |
