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Development of two in vitro regeneration systems through leaf explant and callus culture and the application for genetic transformation in Alstroemeria
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 1998 |
| Abstract | A two-step protocol for the induction of shoots from Alstroemeria leaf expiants has been developed. Leaf expiants with stem node tissue attached were incubated on shoot induction medium for 10 days, and then transferred to regeneration medium. Shoots from the area adjacent to the region between leaf base and node tissue regenerated within three weeks after transfer to the regeneration medium, without a callus phase. The best induction was obtained with Murashige and Skoog (MS) medium containing 10 |aM thidiazuron (TDZ) and 0.5 uM indole butyric acid (IBA). The regeneration medium contained 2.2 uM 6benzylaminopurine (BAP). After several subcultures of the leaf expiants with induced shoots, normal plantlets with rhizome were formed. In Alstroemeria the percentage of responding leaf expiants is more important than the number of shoots regenerated per leaf expiant, because rhizome formation is the most important factor for micropropagation. The effect of other compounds in the induction medium, including glucose, sucrose, silver nitrate, and ancymidol, on regeneration was also investigated. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://edepot.wur.nl/210290 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
