NDLI: The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

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The effect of hygromycin on regeneration in different Alstroemeria explant types after Agrobacterium-mediated transformation

Content Provider	Semantic Scholar
Author	Kim, Ju Byoung Jeu, Marjo J. De Raemakers, C. J. J. M. Jacobsen, Evert Visser, Richard G. F.
Copyright Year	2002
Abstract	This is the first successful report of Agrobacterium-mediated transformation in Alstroemeria by infection of FEC (Friable Embryogenic Callus) lines and leaves with axil tissues. Of the transformation methods, particle bombardment and Agrobacterium-mediated transformation have been widely used to transfer foreign DNA into the plant genome. Especially, Agrobacterium tumefaciens efficiently infects most plants. Most monocotyledonous plants, including Alstroemeria, are recalcitrant to A. tumefaciens infection. Therefore, it is essential to develop an efficient, reliable protocol for Agrobacterium-mediated transformation in Alstroemeria. In order to achieve this aim, different hygromycin concentrations in selection medium were tested and compared in FEC explants and leaves with axil tissues using Agrobacterium strain LBA4404 (pTOK233). As a result, 20 mg/l hygromycin was a proper concentration to select for both FEC lines and leaves with axil tissues. Using this concentration, transformed lines were obtained. Moreover, FEC lines showed at least 2.5 times higher rate in the survival rate, and 5 times higher rate in transient GUS gene expression than those of leaves with axil tissues. The protocol, seems to be a promising method for the transformation of the monocot Alstroemeria with genes of interest in the near future.
Starting Page	97
Ending Page	103
Page Count	7
File Format	PDF HTM / HTML
DOI	10.17660/ActaHortic.2002.572.11
Volume Number	572
Alternate Webpage(s)	http://library.wur.nl/WebQuery/wurpubs/fulltext/18477
Alternate Webpage(s)	https://doi.org/10.17660/ActaHortic.2002.572.11
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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