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An Approach to Identifying the Functionally Important RNA Sites by Complementary Addressed Modification
| Content Provider | Semantic Scholar |
|---|---|
| Author | Malygin, Alexey A. Graifer, Dmitri M. Laletina, Elena Shatsky, Ivan N. Karpova, Galina G. |
| Copyright Year | 2004 |
| Abstract | An approach based on complementary addressed modification of nucleic acids by oligodeoxyribonucleotide derivatives was proposed for changing the spatial structure of particular RNA sites in order to study their role in the biological activity of the total RNA molecule. Hepatitis C virus (HCV) IRES was used as a model. Oligodeoxyribonucleotide derivatives contained a 4-[N-(2-chloroethyl)-N-methylamino]benzylamino group at the 5′-phosphate and were complementary to various RNA sites located in regions of hairpins II, IIId, or IIIe. Covalent adducts resulting from RNA alkylation with the derivatives were isolated by denaturing PAGE and tested for binding with the 40S subunit of human ribosomes. Structural alteration of hairpin II had no effect on the binding, whereas that of hairpin IIIe substantially reduced it. The RNA with modified hairpin IIId showed virtually no binding with the 40S subunit. Hairpin IIId was assumed to play the critical role in the binding of HCV IRES with the 40S subunit. |
| Starting Page | 873 |
| Ending Page | 879 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1023/B:MBIL.0000008356.03043.9a |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1023/B:MBIL.0000008356.03043.9a |
| Alternate Webpage(s) | https://doi.org/10.1023/B%3AMBIL.0000008356.03043.9a |
| Volume Number | 37 |
| Journal | Molecular Biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
