NDLI: An Approach to Identifying the Functionally Important RNA Sites by Complementary Addressed Modification

Content Provider	Springer Nature Link
Author	Malygin, A. A. Graifer, D. M. Laletina, E. S. Shatsky, I. N. Karpova, G. G.
Copyright Year	2003
Abstract	An approach based on complementary addressed modification of nucleic acids by oligodeoxyribonucleotide derivatives was proposed for changing the spatial structure of particular RNA sites in order to study their role in the biological activity of the total RNA molecule. Hepatitis C virus (HCV) IRES was used as a model. Oligodeoxyribonucleotide derivatives contained a 4-[N-(2-chloroethyl)-N-methylamino]benzylamino group at the 5′-phosphate and were complementary to various RNA sites located in regions of hairpins II, IIId, or IIIe. Covalent adducts resulting from RNA alkylation with the derivatives were isolated by denaturing PAGE and tested for binding with the 40S subunit of human ribosomes. Structural alteration of hairpin II had no effect on the binding, whereas that of hairpin IIIe substantially reduced it. The RNA with modified hairpin IIId showed virtually no binding with the 40S subunit. Hairpin IIId was assumed to play the critical role in the binding of HCV IRES with the 40S subunit.
Starting Page	873
Ending Page	879
Page Count	7
File Format	PDF
ISSN	00268933
Journal	Molecular Biology
Volume Number	37
Issue Number	6
e-ISSN	16083245
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Human Genetics Life Sciences Biochemistry
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biophysics

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An Approach to Identifying the Functionally Important RNA Sites by Complementary Addressed Modification

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