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RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bolinger, Cheryl Yilmaz, Alper Hartman, Tiffiney Roberts Kovacic, Melinda Butsch Ye, Jianxin Forget, Mary Boris-Lawrie, Kathleen |
| Copyright Year | 2007 |
| Abstract | The 5' untranslated region (UTR) of retroviruses contain structured replication motifs that impose barriers to efficient ribosome scanning. Two RNA structural motifs that facilitate efficient translation initiation despite a complex 5' UTR are internal ribosome entry site (IRES) and 5' proximal post-transcriptional control element (PCE). Here, stringent RNA and protein analyses determined the 5' UTR of spleen necrosis virus (SNV), reticuloendotheliosis virus A (REV-A) and human T-cell leukemia virus type 1 (HTLV-1) exhibit PCE activity, but not IRES activity. Assessment of SNV translation initiation in the natural context of the provirus determined that SNV is reliant on a cap-dependent initiation mechanism. Experiments with siRNAs identified that REV-A and HTLV-1 PCE modulate post-transcriptional gene expression through interaction with host RNA helicase A (RHA). Analysis of hybrid SNV/HTLV-1 proviruses determined SNV PCE facilitates Rex/Rex responsive element-independent Gag production and interaction with RHA is necessary. Ribosomal profile analyses determined that RHA is necessary for polysome association of HTLV-1 gag and provide direct evidence that RHA is necessary for efficient HTLV-1 replication. We conclude that PCE/RHA is an important translation regulatory axis of multiple lymphotropic retroviruses. We speculate divergent retroviruses have evolved a convergent RNA-protein interaction to modulate translation of their highly structured mRNA. |
| Starting Page | 2629 |
| Ending Page | 2642 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 17426138v1 |
| Volume Number | 35 |
| Journal | Nucleic acids research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 5' Untranslated Regions Cellular Phone DHX9 gene Gene Expression Genetic Translation Process Human T-lymphotropic virus 1 Internal Ribosome Entry Sites Leukemia, B-Cell Leukemia, T-Cell Post-Transcriptional Regulation Proviruses RNA Helicase Reticuloendotheliosis virus Reticuloendotheliosis, X-linked Retroviridae Ribosomes Spleen Tissue T-Lymphocyte Trager duck spleen necrosis virus Transcription Initiation Transcription, Genetic Transcriptional Regulation Translation Initiation leukemia |
| Content Type | Text |
| Resource Type | Article |
