NDLI: Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey.

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Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey.

Content Provider	Semantic Scholar
Author	Jeong, Seok Hoon Bae, Il Kwon Lee, Jung Hun Sohn, Seung Ghyu Kang, Geun Kim, Young Ho Jeong, Byeong Chul Lee, Sang Hee
Copyright Year	2004
Abstract	To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producing isolates were detected in every region in Korea. A total of 44.6% of the isolates produced both TEM- and SHV-type ESBLs, and 52% of ESBL-producing isolates transferred resistance to ceftazidime by transconjugation. The ESBLs were TEM-19, TEM-20, TEM-52, SHV-2a, SHV-12, and one new variant identified for the first time in Korea, namely, TEM-116. TEM-1 and SHV-12 were by far the most common variants. TEM-1, TEM-116, and SHV-12 showed a high prevalence in K. pneumoniae. Two isolates (E. coli SH16 and K. pneumoniae SV3) produced CMY-1-like beta-lactamases, which play a decisive role in resistance to cefoxitin and cefotetan, as well as TEM-type enzymes (TEM-20 and TEM-52, respectively). Using MIC patterns and DNA sequencing analysis, we postulated a possible evolution scheme among TEM-type beta-lactamases in Korea: from TEM-1 to TEM-19, from TEM-19 to TEM-20, and from TEM-20 to TEM-52.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jcm.asm.org/content/42/7/2902.full.pdf
PubMed reference number	15243036v1
Volume Number	42
Issue Number	7
Journal	Journal of clinical microbiology
Language	English
Access Restriction	Open
Subject Keyword	Academic Medical Centers Biopolymer Sequencing Cefotetan Cefoxitin Ceftazidime Klebsiella pneumoniae Pneumonia Sequence Analysis beta-lactamase SHV-2a
Content Type	Text
Resource Type	Article

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