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Effects of cholesterol and unsaturated DOPC lipid on chain packing of saturated gel-phase DPPC bilayers.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mills, Thalia T. Huang, Juyang Feigenson, Gerald W. Nagle, John F. |
| Copyright Year | 2009 |
| Abstract | Wide angle x-ray scattering (WAXS) from oriented lipid multilayers was used to study the effect of adding cholesterol (Chol) or 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) to gel-phase 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers. Small quantities (X < 0.10 mole fraction) of both molecules disrupt the tight packing of tilted chains of pure gel-phase DPPC, forming a more disordered, untilted phase. The addition of larger quantities of DOPC causes the sample to phase-separate into a gel-phase, characterized by a narrow WAXS peak, and liquid disordered phase, characterized by wide, diffuse WAXS scattering. In contrast, two WAXS peaks indicative of two coexisting phases were not observed in Chol/DPPC mixtures (X(Chol) = 0.07 to 0.40). Instead, Chol caused a gradual increase in the width of the WAXS peak, consistent with a gradual change from a more gel-like to a more liquid-like state rather than passing through a region of two phase coexistence. Our WAXS data include a huge amount of information. A new method of analysis suggests that WAXS data may provide definitive results relating to the disagreements between previously published phase diagrams for Chol/DPPC. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 19592709 |
| Journal | Medline |
| Volume Number | 28 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://lipid.phys.cmu.edu/papers09/ProofsMills.pdf |
| Alternate Webpage(s) | http://lipid.phys.cmu.edu/papers09/GPB_Mills09.pdf |
| Alternate Webpage(s) | http://feigensonlab.mbg.cornell.edu/publications/Mills2009.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.bpj.2008.12.3198 |
| Journal | General physiology and biophysics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
