NDLI: Coexisting stripe- and patch-shaped domains in giant unilamellar vesicles (2006)

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Coexisting stripe- and patch-shaped domains in giant unilamellar vesicles (2006)

Content Provider	CiteSeerX
Author	Li, Li Cheng, Ji-Xin
Abstract	ABSTRACT: We report a new type of gel-liquid phase segregation in giant unilamellar vesicles (GUVs) of mixed lipids. Coexisting patch- and stripe-shaped gel domains in GUV bilayers composed of DOPC/ DPPC or DLPC/DPPC are observed by confocal fluorescence microscopy. The lipids in stripe domains are shown to be tilted according to the DiIC18 fluorescence intensity dependence on the excitation polarization. The patch domains are found to be mainly composed of DPPC-d62 according to the coherent anti-Stokes Raman scattering (CARS) images of DOPC/DPPC-d62 bilayers. When cooling GUVs from above the miscibility temperature, the patch domains start to appear between the chain melting and the pretransition temperature of DPPC. In GUVs containing a high molar percentage of DPPC, the stripe domains form below the pretransition temperature. Our observations suggest that the patch and stripe domains are in the Pâ ′ and Lâ ′ gel phases, respectively. According to the thermoelastic properties of GUVs described by Needham and Evans [(1988) Biochemistry 27, 8261-8269], the Pâ ′ and Lâ ′ phases are formed at relatively low and high membrane tensions, respectively. GUVs with high DPPC percentage have high membrane surface tension and thus mainly exhibit Lâ ′ domains, while GUVs with low DPPC percentage have low membrane surface tension and form Pâ ′ domains accordingly. Adding negatively charged lipid to the lipid mixtures or applying an osmotic pressure to GUVs using sucrose solutions releases the surface
File Format	PDF
Journal	Biochemistry
Language	English
Publisher Date	2006-01-01
Access Restriction	Open
Subject Keyword	Giant Unilamellar Vesicle Patch-shaped Domain Pretransition Temperature Stripe Domain Gel-liquid Phase Segregation Diic18 Fluorescence Intensity Dependence High Dppc Percentage Chain Melting Mixed Lipid Dlpc Dppc Stripe-shaped Gel Domain Patch Domain Sucrose Solution Confocal Fluorescence Microscopy Gel Phase Guv Bilayers Miscibility Temperature Low Dppc Percentage Dopc Dppc Exhibit Domain High Membrane Tension Osmotic Pressure Dopc Dppc-d62 Bilayers Excitation Polarization Thermoelastic Property New Type High Molar Percentage Coherent Anti-stokes Raman Scattering Low Membrane Surface Tension High Membrane Surface Tension Lipid Mixture
Content Type	Text
Resource Type	Article

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