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Targeting effect of microRNA on CD133 and its impact analysis on proliferation and invasion of glioma cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhao, Chunbo Ma, Zhiguo Mou, Shanling Yang, Yonglin Zhang, Yunyan Yao, Wei-cheng |
| Copyright Year | 2017 |
| Abstract | MiR-200b, a member of the microRNA-200 family, has been identified to be capable of suppressing glioma cell growth through targeting CREB1 or CD133. However, whether miR-200b affects the biological behavior (proliferation, invasion, and migration) of glioma cells is poorly understood. The aim of this study was to evaluate the effect of miR-200b on the biological behavior of glioma cells in vitro. MiRNA-200b mimics, miRNA-200b inhibitor, and mimic control were transfected into conventionally cultured glioma U251 cells, followed by measuring the expression of miR-200b and CD133 in transfected cells by RT-PCR; effect of miR-200b on CD133 mRNA 3'-UTR luciferase activity by luciferase reporter assay; proliferation activity of transfected U251 cells by MTT method; and changes in U251 cell invasion and migration by Transwell method after transfection. Compared to that in the miRNA-200b inhibitor, mimic control, and blank control groups, miRNA-200b expression was significantly increased and CD133 mRNA expression was significantly decreased in the mimic miRNA-200b group in a time-dependent manner (P < 0.05). Meanwhile, dual luciferase reporter assay showed that miR-200b could inhibit CD133 activity through binding to the 3'-UTR of CD133 mRNA (P < 0.05). Furthermore, the proliferation activity and invasion and migration abilities of U251 cells transfected with miRNA-200b mimic were significantly decreased (P < 0.05). In conclusion, overexpression of miR-200b inhibited the proliferation, invasion, and migration of glioma cells possibly through targeting CD133. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.funpecrp.com.br/gmr/year2017/vol16-1/pdf/gmr-16-01-gmr.16019281.pdf |
| PubMed reference number | 28362995v1 |
| Alternate Webpage(s) | https://doi.org/10.4238/gmr16019281 |
| DOI | 10.4238/gmr16019281 |
| Journal | Genetics and molecular research : GMR |
| Volume Number | 16 |
| Issue Number | 1 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Dual Glioma Luciferases MicroRNAs Reverse Transcriptase Polymerase Chain Reaction U251 cell growth cellular targeting luciferin monooxygenase activity monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
